Re‐Programming and Optimization of a L ‐Proline cis ‐ 4‐Hydroxylase for the cis ‐3‐Halogenation of its Native Substrate

The Front Cover represents the discovery of a novel path during scientist′s journey towards biocatalytic incorporation chlorine into L-proline. In their Communication, A. Papadopoulou et al describe remodeling an Fe/αKG hydroxylase halogenase utilizing tools protein engineering. By employing several rounds directed evolution, remodeled variant with 98-fold improved apparent kcat/Km was generated. reprogrammed biocatalyst is first example stereo- and regioselective derivatizing L-proline at C3 position highlights possibility to successfully access diverse chemistries Fe/αKG-dependent dioxygenases expand toolbox. More information can be found in Communication by al.